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KMID : 0380020130280010013
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 1 p.13 ~ p.17
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Anti-Inflammatory Effect of Chloroform Extract from Potentilla chinensis
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Kang Chang-Ho
Han Sang-Hyun
So Jae-Seong
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Abstract
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In this study, we investigated the anti-inflammation effect of Potentilla chinensis (PC) on Raw264.7 macrophage cells. Ethanol extract of PC decreased the production of nitric oxide (NO) in LPS-stimulated RAW264.7 cells. Ethanol extract was fractioned by n-hexane, chloroform, ethyl acetate, n-butanol, water and each fraction was tested for inhibitory effects on inflammation. Among the sequential solvent fractions, PC chloroform extracts (50, 100, 300, and 500 μg/mL) significantly suppressed LPS-stimulated production of NO. During the entire experimental period, 200 and 300 μg/mL of PC chloroform extracts had no cytotoxicity. LPS-induced NO and prostaglandin E2 (PGE2) production were inhibited by PC chloroform extracts up to 50% and 90% of these productions, respectively. PC chloroform extracts reduced the expression of iNOS and COX-2 gene. These results suggest that PC chloroform extracts exhibit strong effects of anti-inflammation and can be a potential candidate in the treatment of acute and chronic inflammatory diseases.
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KEYWORD
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Potentilla chinensis, Nitric oxide, Prostaglandin E2, iNOS, COX-2
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